| Annotated protein: | F-box/SPRY domain-containing protein 1 (F-box only protein 45) (mFbxo45). Gene symbol: FBXO45. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8K3B1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ FBXO45 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle exocytosis (GO:2000300) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Tada H, et al. "Fbxo45, a novel ubiquitin ligase, regulates synaptic activity" J Biol Chem. 2010 Feb 5;285(6):3840-3849 PMID:19996097 |
| Figure(s): | Figure 4 |
| Annotation description: | siRNA-mediated knockdown of Fbxo45 significantly increased mEPSC frequency without changing its amplitude. Overexpression of Fbxo45 decreased the mEPSC frequency. Neither affected the number of synapses suggesting that Fbxo45 regulates neurotransmission machinery. Fbxo45 interacts with Skp1 and induces protein ubiquitination. Fbxo45 binds Munc13-1 and Fbxo45 over expression reduces Munc13-1 levels while siRNA mediated Fbxo45 knockdown delays Munc13-1 degradation suggesting that Fbxo45 controls synaptic vesicle fusion via Muc13-1. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Ruud Toonen (ORCID:0000-0002-9900-4233) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3716 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |