| Annotated protein: | Numb-like protein. Gene symbol: NUMBL. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A1L1I3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NUMBL |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Nishimura T, et al. "Role of numb in dendritic spine development with a Cdc42 GEF intersectin and EphB2" Mol Biol Cell. 2006 Mar;17(3):1273-85 PMID:16394100 |
| Figure(s): | Fig.2, 7 |
| Annotation description: | Fig.2: "Suppression of Numb affects spine development" - "Quantitative analysis showed that the suppression of Numb and Numb-like resulted in a decrease in the number of dendritic protrusions (p < 0.005, siScramble 4.25±0.24 per 10 μm of dendrites [mean ± SEM], n = 35, siNumb and siNumb-like 3.13 ± 0.19, n = 29) and in a decrease in the length of remaining protrusions (p < 0.005, siScramble 1.74 ± 0.02 μm, n = 860; siNumb and siNumb-like 1.53 ± 0.02 μm, n = 871; Figure 2, E and F). " Fig.7: "Suppression of Numb impairs ephrin-B1-induced spine development and maturation" - "Treatment with clustered ephrin-B activates Cdc42 and thereby results in an increase in the number and size of dendritic protrusions (Irie and Yamaguchi, 2002 blue right-pointing triangle; Henkemeyer et al., 2003 blue right-pointing triangle; Penzes et al., 2003 blue right-pointing triangle). Some were long and thin protrusions, resembling filopodia, some were short and wide protrusions, and others had necks and mushroom-shaped heads, resembling mature spines (Figure 7C). Quantitative analysis indicated that ephrin-B1 increased the number of dendritic protrusions and PSD95-containing spines with mushroom-shaped heads (Figure 7, D and E). Suppression of Numb proteins by siRNAs partially impaired the ephrin-B1-induced protrusion formation. Suppression of Numb reduced the percentage of the protrusions with mushroom-shaped head (siScramble, 67 ± 3.5%; siNumb and siNumb-like, 57 ± 4.1%, p < 0.05) and also reduced the percentage of PSD95-positive spines per total spines (Figure 7E). Taken together, these results suggest that Numb controls synaptic development and maturation both upstream and downstream of ephrin-B1, presumably with intersectin and Cdc42." 17/3/2018 Pim - Note: in Fig.5 the authors show that Numb regulates the GEF activity of CDC42. The effects of Numb on spine morphology are presumably (in part) via actin remodelling. However, since not show in situ, I have not annotated to this more specific term. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Cell-free system |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3707 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |