| Annotated protein: | Rho GTPase-activating protein 44 (Rho-type GTPase-activating protein RICH2) (RhoGAP interacting with CIP4 homologs protein 2) (RICH-2). Gene symbol: ARHGAP44. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q5SSM3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARHGAP44 |
| Ontology domain: | Biological Process |
| SynGO term: | modulation of chemical synaptic transmission (GO:0050804) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Raynaud F, et al. "Shank3-Rich2 interaction regulates AMPA receptor recycling and synaptic long-term potentiation" J Neurosci. 2013 Jun 5;33(23):9699-715 PMID:23739967 |
| Figure(s): | figure 3-5 |
| Annotation description: | Fig.3B/C Rich2 KD (shRNA) decreases transferrin-rhodamine recycling in neurons and COS-7 cells [fluorescent pulse-chase]. Fig.3D Rich2 promotes recycling of GFP-tagged AMPARs to the surface of hipp neurons Fig.4/5 Rich2 activity is needed for spine enlargement induced by chemical LTP and increase of synaptic currents, and increase of surface levels of synaptic AMPARs [fixed tissue] (NMDAR activation) [Rich2 KD, OE Rich2 mutant proteins] 11/12/2017 Pim - Since ARHGAP44 / Rich2 is a regulator of signaling and not a transporting protein itself I have selected the "regulates..." variant of the term. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3703 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |