| Annotated protein: | Gephyrin (Putative glycine receptor-tubulin linker protein) [Includes: Molybdopterin adenylyltransferase (MPT adenylyltransferase) (EC 2.7.7.75) (Domain G); Molybdopterin molybdenumtransferase (MPT Mo-transferase) (EC 2.10.1.1) (Domain E)]. Gene symbol: GPHN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q03555 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GPHN |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0099645) |
| Synapse type(s): | spinal cord, glycinergic |
| Annotated paper: | Kirsch J, et al. "Gephyrin antisense oligonucleotides prevent glycine receptor clustering in spinal neurons" Nature. 1993 Dec 23-30;366(6457):745-8 PMID:8264797 |
| Figure(s): | Fig. 4, Fig. 2 |
| Annotation description: | Gephyrin is essential for the postsynaptic localization of glycine receptors (GlyR). Antisense knock-down of Gephyrin expression suppresses clustering of GlyR. 29/3/2017 Pim - The experiment shows GlyR clusters at the membrane as opposed to intracellular GlyR hotspots. The membrane GlyR clusters are inferred to reflect GlycR clusters in the postsynaptic membrane. - The GlyR hotspots are presumed to be synaptic receptors by the authors and a er opposed to GPHN which is a marker for a postsynaptic specialization (see feedback thread). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| Additional literature: | This paper describes the direct physical interaction of Gephyrin with Glycine receptor beta-subunit. @ PMID:7546736 Disrupted GlyR clustering is also observed in Gephyrin KO mice (Fig. 3). @ PMID:9812897 |
| SynGO annotation ID: | 369 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |