| Annotated protein: | Disks large-associated protein 1 (DAP-1) (Guanylate kinase-associated protein) (rGKAP) (PSD-95/SAP90-binding protein 1) (SAP90/PSD-95-associated protein 1) (SAPAP1). Gene symbol: DLGAP1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P97836 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DLGAP1 |
| Ontology domain: | Biological Process |
| SynGO term: | modulation of chemical synaptic transmission (GO:0050804) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Moutin E, et al. "GKAP-DLC2 interaction organizes the postsynaptic scaffold complex to enhance synaptic NMDA receptor activity" J Cell Sci. 2012 Apr 15;125(Pt 8):2030-40 PMID:22328512 |
| Figure(s): | fig 3; Fig.5; fig 6 |
| Annotation description: | Fig 3 Neuronal activity potentiates GLKAP-DLG2 interaction Literal: "We next analyzed the effect of neuronal activity on GKAP-DLC2 interaction. To enhance neuronal activity, we used a specific blocker of transient outward voltage-dependent potassium currents, 4-AP (1 mM. BRET signal between RLuc8- GKAP and Venus-DLC2 in hippocampal neurons was recorded before and during 4-AP exposure (Fig. 3A)."After 4-AP exposure, the mean BRET signal increased in soma and dendrites" Fig 6 GKAP-DLC2 interaction enhances NMDA synaptic currents Literal "Because GKAP-DLC2 interaction promotes synaptic localizationb of PSD-95, we investigated the modulatory role of this protein assembly on endogenous NMDA current. Neurons transfected with CFP-GKAP or CFP-GKAPmut were recorded in the whole-cell configuration using the patch-clamp technique and transiently perfused with NMDA. GKAP induced a increase in NMDA currents. " We further studied the modulatory action of the GKAP-DLC2 interaction on NMDA current at the synaptic level by analyzing the slow component of miniature excitatory postsynaptic currents (mEPSCs) carried by NMDA currents. The NMDA component was measured 7 mseconds after the peak amplitude of averaged mEPSCs, in untransfected neurons or neurons transfected with GKAP or GKAPmut. Overexpression of GKAP significantly (P,0.05) increased the amplitude of the synaptic NMDA component." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Protein-protein interaction (generic) |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 3688 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |