| Annotated protein: | Voltage-dependent N-type calcium channel subunit alpha-1B (Brain calcium channel III) (BIII) (Calcium channel, L type, alpha-1 polypeptide isoform 5) (Voltage-gated calcium channel subunit alpha Cav2.2). Gene symbol: CACNA1B. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q02294 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNA1B |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of calcium-dependent activation of synaptic vesicle fusion (GO:0150037) |
| Synapse type(s): | hippocampus, glutamatergic Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Wheeler DB, et al. "Roles of N-type and Q-type Ca2+ channels in supporting hippocampal synaptic transmission" Science. 1994 Apr 1;264(5155):107-11 PMID:7832825 |
| Figure(s): | Fig 1A, B |
| Annotation description: | Transverse hippocampal slices (400 micromolar) were submerged and continuously superfused with Krebs. Synaptic transmission was elicited and field EPSPs (excitatory post synaptic potentials) were recorded with 1- to 3-Mohm pipettes filled with 3 M NaCI. Synaptic strength was monitored as maximal slope of the field EPSP recorded in hippocampal area CA1 during 0.033-Hz stimulation of the Schaffer collateral and commisural pathways. Figs 1A, B show the contribution of Cav2.2 (N-type) to synaptic vesicle exocytosis by demonstrating that a specific blocker of N-type channels (omega-Conotoxin-GVIA) causes a rapid (but incomplete) depression of synaptic transmission. 16/5/2017 Pim - N-type calcium channels are typified by the CACNA1B subunit. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Field recordings |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| Additional literature: | Paper provides evidence that omega-Conotoxin-GVIA (omega-CTX-GVIA) binds specifically to N-type voltage gated calcium channels but not to L-type. They demonstrated that binding of omega-CTX-GVIA to rat forebrain membranes is saturable with a single binding site of low picomolar affinity (Fig 2). Iodinated omega-CTX-GVIA was used @ PMID:2845019 |
| SynGO annotation ID: | 3662 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |