| Annotated protein: | RIMS-binding protein 2 (RIM-BP2). Gene symbol: RIMBP2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q80U40 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RIMBP2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of calcium-dependent activation of synaptic vesicle fusion (GO:0150037) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Grauel MK, et al. "RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses" Proc Natl Acad Sci U S A. 2016 Oct 11;113(41):11615-11620 PMID:27671655 |
| Figure(s): | Figure 2-4 |
| Annotation description: | It is demonstrated that RIM-BP2 clusters calcium channels to the active zone. The authors use STED microscopy and an antibody against Cav2.1 to show that genetic deletion of RIM-BP2 increases the distance between active zone markers and calcium channels, and electrophysiology to show a modest reduction in release probability. This is in line with findings in the fly NMJ, where removal of RIM-BP leads to a reduction in calcium channel density at the active zone. 3/2/2017 Pim - effect on Ca2+ sensitivity of SV release in Fig.3 (no effect found on global Ca2+-concentration) - effect on VGCC localization shown in Fig.4 - effect on synaptic transmission (STP) shown in Fig.2 (and 3) |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution Optical physiology |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | Liu at al. showed that RIMBP knockout in drosophila has a similar phenotype. @ PMID:22174254 Acuna et.al. used a RIMBP1/2 double knockout and found a similar phenotype, although it proposes a different working model. @ PMID:26402606 |
| SynGO annotation ID: | 3654 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |