| Annotated protein: | Voltage-dependent L-type calcium channel subunit beta-1 (CAB1) (Calcium channel voltage-dependent subunit beta 1). Gene symbol: CACNB1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8R3Z5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNB1 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Obermair GJ, et al. "Reciprocal interactions regulate targeting of calcium channel beta subunits and membrane expression of alpha1 subunits in cultured hippocampal neurons" J Biol Chem. 2010 Feb 19;285(8):5776-91 PMID:19996312 |
| Figure(s): | Fig 4a |
| Annotation description: | Primary hippocampal neurons were fixed and stained with mouse monoclonal anti-beta1 antibody. anti-beta1 staining could be detected in the soma and dendrites (Fig. 1B). The staining appeared punctate along dendrites (Fig. 1C) and showed partial co-localization with antibody staining for a synaptic marker (synapsin) although the majority of beta1 clusters appeared to be adjacent to synapsin clusters. Object-based colocalization analysis of 24 DIV neurons was also used to support partial overlap of beta1 subunits with synapsin (% of β clusters colocalized with synapsin ± 95% confidence intervals (n): β1, 46 ± 4 (21). Although beta 1 expression in axons is lower than some of the other subunits it can be detected. This data shows that some beta1 is axonal and presynaptic but this is a minority of the protein. Expression of tagged beta1 that provides better resolution shows clear overalap with presynaptic markers (Fig 4) Supplementary Fig 1. Specificity of anti-beta antibodies tested. Dysgenic myotubes were transfected with a V5 epitope containing version of the beta1 subunit. Cells were fixed and stained with antibodies against all 4 subunits but the antibodies did not label non-transfected neighboring cells, or cross-react with cells expressing any of the other beta isoforms (Suppl. Fig. 1C). 21/11/2017 Pim - literal: "Thus, β subunits display an isoform-specific expression in the axonal compartment in that β2, β3, and β4 isoforms are expressed throughout the axon, whereas β1a and β1b are largely excluded from the distal axon and small axonal branches." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| SynGO annotation ID: | 363 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |