Annotated protein:	Alpha-soluble NSF attachment protein (SNAP-alpha) (N-ethylmaleimide-sensitive factor attachment protein alpha). Gene symbol: NAPA. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9DB05
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SynGO gene info:	SynGO data @ NAPA
Ontology domain:	Biological Process
SynGO term:	synaptic vesicle priming (GO:0016082)
Synapse type(s):	hippocampus, glutamatergic
Annotated paper:	Burgalossi A, et al. "SNARE protein recycling by alphaSNAP and betaSNAP supports synaptic vesicle priming" Neuron. 2010 Nov 4;68(3):473-87 PMID:21040848
Figure(s):	Fig 5-7
Annotation description:	Double mutant mice of (i) hypomorph HYH (recessive missense mutation (M105I)in alpha-SNAP)a dn (ii) beta-SNAP KO show 25% reduced RRP size as determined by HSS stimulation without changes in evoked EPSC amplitudes or mEPSC amplitudes and frequencies. EPSC recovery after electrical discharge of the RRP by long 40 Hz and 100 Hz AP trains was slower in DKO neurons as compared to Ctrl cells. 8/12/2016 Pim - hypomorph HYH reduces the amount of aSNAP and is embryonically lethal - Syn.Tr. in hypomorph HYH aSNAP mouse is unaltered. - Syn.Tr. in bSNAP mouse is unaltered. - aSNAP and bSNAP are functionally interchangable (also shown in Fig 7) - In the Dmut mouse bSNAP is completely removed and aSNAP is present at reduced levels - The results in the Dmut mouse should be attributed to reduction of aSNAP
Evidence tracking, Biological System:	Cultured neurons
Evidence tracking, Protein Targeting:	Genetic transformation (eg; knockout, knockin, mutations)
Evidence tracking, Experiment Assay:	Whole-cell patch clamp
Annotator(s):	Ruud Toonen (ORCID:0000-0002-9900-4233) Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:	Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:	35
Dataset release (version):	20231201
View annotation as GO-CAM model: