| Annotated protein: | Voltage-dependent calcium channel subunit alpha-2/delta-1 (Voltage-gated calcium channel subunit alpha-2/delta-1) [Cleaved into: Voltage-dependent calcium channel subunit alpha-2-1; Voltage-dependent calcium channel subunit delta-1]. Gene symbol: CACNA2D1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P54290 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNA2D1 |
| Ontology domain: | Cellular Component |
| SynGO term: | anchored component of presynaptic active zone membrane (GO:0099029) |
| Synapse type(s): | spinal cord, glutamatergic |
| Annotated paper: | Bauer CS, et al. "The increased trafficking of the calcium channel subunit alpha2delta-1 to presynaptic terminals in neuropathic pain is inhibited by the alpha2delta ligand pregabalin" J Neurosci. 2009 Apr 1;29(13):4076-88 PMID:19339603 |
| Figure(s): | Fig 1E, F |
| Annotation description: | Preembedding immunogold electron microscopy (EM) on lamina I-III of L4-L6 spinal cord sections was used to study the subcellular localization of alpha_2 delta-1. Alpha_2 delta-1 was observed at the plasma membrane of presynaptic nerve terminals of DRG neurons, at the plasma membrane of postsynaptic dorsal horn neurons and also at intracellular dendritic sites (Fig 1E, F). 27/4/2017 Pim - In discussion with the expert we have establised that PMID:20080692 (Fig.4G) shows that the Ca-channel alpha_2 delta-1 subunit may have a TM domain predicted, but that A GPI anchor provides most likely the link to the plasma membrane. We have selected the term "anchored component of presynaptic active zone membrane (GO:0099029)" accordingly. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| Additional literature: | Paraffin-embedded sections of rat brain were stained with primary antibody specific for alpha_2 delta-1 and a horseradish peroxidase coupled secondary. The alpha_2 delta-1 protein was observed at the neuropil, and at a much lower level in somata (Fig 6). @ PMID:18616987 Provides evidence that alpha_2 delta-1 is GPI anchored in the plasma membrane of cells. alpha_2 delta-1 is expressed on the plasma membrane of DRGs in culture (Fig. 4E, immunofluorescence) and incubation with phosphatidylinositol-phospholipase C (PI-PLC) causes it to be cleaved from the surface of cells. Alpha_2 delta-1 from hippocampus also partitions into cholesterol-rich detergent-resistant membrane (DRM) fractions - termed 'lipid rafts'. @ PMID:20080692 Fig S4 Used antibody labeling and fluorescence microscopy to observe alpha_2 delta-1 at hippocampal synapses @ PMID:22678293 |
| SynGO annotation ID: | 344 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |