| Annotated protein: | Ephrin-B2 (ELF-2) (EPH-related receptor tyrosine kinase ligand 5) (LERK-5) (HTK ligand) (HTK-L). Gene symbol: EFNB2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P52800 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EFNB2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Essmann CL, et al. "Serine phosphorylation of ephrinB2 regulates trafficking of synaptic AMPA receptors" Nat Neurosci. 2008 Sep;11(9):1035-43 PMID:19160501 |
| Figure(s): | Figs. 1, 2, 8 |
| Annotation description: | EphrinB2 activation by EphB4 inhibits AMPA-induced AMPA receptor internalization. Fig 1. AMPA receptor surface expression and internalization were monitored by an antibody feeding assay and fluorescence microscopy, and a biotinylation assay with western blotting. Activation of ephrinB2 by feeding cultured hippocampal neurons pre-clustered EphB4-Fc enhanced AMPA-induced surface expression of AMPA receptors and thereby inhibited AMPA receptor internalization. Fig 2. Hippocampal neurons from ephrinB2 knockout mice showed enhanced AMPA receptor internalization as monitored by an antibody feeding assay, and smaller miniature EPSCs Fig 8. WT ehprinB2-CFP, but not phospho-mutant ephrinB2-CFP, normalized AMPA receptor surface expression in cultured hippocampal neurons as monitored by an antibody feeding assay. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Wide-field fluorescence Whole-cell patch clamp Western blot IP + WB/MSMS |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| SynGO annotation ID: | 341 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |