| Annotated protein: | Synapsin-1 (Synapsin I). Gene symbol: SYN1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P09951 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SYN1 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of synaptic vesicle membrane (GO:0098850) |
| Synapse type(s): | brain |
| Annotated paper: | Huttner WB, et al. "Synapsin I (protein I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic vesicles studied in a highly purified synaptic vesicle preparation" J Cell Biol. 1983 May;96(5):1374-88 PMID:6404912 |
| Figure(s): | Fig. 7 |
| Annotation description: | In this study, Huttner et al. isolated synaptic vesicles (SVs) from rat brain to virtual homogeneity (Fig. 6). Using an antibody specific for synapsin 1, a high enrichment of the protein in the purified SV fraction was observed (Figs. 3, 4). The interaction between synapsin and synaptic vesicles was abolished when the ionic strength of the medium was increased, suggesting an electrostatic component in the membrane association (Fig. 9). Also, they showed that phosphorylation of synapsin at the domain involved in the binding to synaptic vesicles decreases the binding between synapsin I and synaptic vesicles (Figs. 11-13). 5/2/2018 Pim - Synapsin 1 has a region that binds phospholipids with a preference for negatively charged species. For this reason the 'extrinsic component ...' variant was selected. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | In this paper, De Camilli P., et al. investigated the subcellular localization of synapsin1 in the nerve terminal using electron microscopy. They found that synapsin 1 is localized in at the cytoplasmic surface of the synaptic vesicles (Figure 3). @ PMID:6404911 |
| SynGO annotation ID: | 317 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |