| Annotated protein: | Semaphorin-4F (Semaphorin-W) (Sema W). Gene symbol: SEMA4F. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z143 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SEMA4F |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Schultze W, et al. "Semaphorin4F interacts with the synapse-associated protein SAP90/PSD-95" J Neurochem. 2001 Aug;78(3):482-9 PMID:11483650 |
| Figure(s): | Fig.2, 3, 4, 5 |
| Annotation description: | Fig.2: "PDZ2 of SAP90/PSD-95 is able to precipitate a chimeric EphrinB1/Sema4F protein" Fig.3: "EphrinB1/Sema4F, but not EphrinB1/Sema4FΔ, is able to translocate SAP90/PSD-95 from the cytosol to the membrane. " Fig.4: "Sema4F immunoreactivity is present in synaptosome fractions and enriched in post-synaptic density fractions from rat brain" Fig.5: "Colocalization of Sema4F with SAP90/PSD-95 and synapsin1 in cultured hippocampal neurons" - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue Cell-free system |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS Y2H |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3131 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |