| Annotated protein: | Neuronal pentraxin-2 (NP2) (Neuronal activity-regulated pentraxin) (Neuronal pentraxin II) (NP-II). Gene symbol: NPTX2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P97738 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NPTX2 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0099645) |
| Synapse type(s): | spinal cord, glutamatergic |
| Annotated paper: | O'Brien RJ, et al. "Synaptic clustering of AMPA receptors by the extracellular immediate-early gene product Narp" Neuron. 1999 Jun;23(2):309-23 PMID:10399937 |
| Figure(s): | Figs. 7, 8, 10 |
| Annotation description: | The secreted protein Narp is enriched at excitatory synapses in cultured neurons (Fig. 3) and present at asymmetric synapses in vivo (Fig. 6). Functionally, surface contact of neurons with non-neuronal cells heterologously expressing Narp induces the clustering of endogenous GluA1 in contacting neurons (Fig. 10). Narp1 also aggregates heterologously expressed GluA1/2/3 on the neuronal cell surface (Fig. 7). In agreement, Narp directly binds to the extracellular domain of AMPA receptors GluA1/2/3 (Fig. 8A) and forms a complex with GluA1 in the brain (Fig. 8B). 13/3/2017 Pim -This annotation does also heavily rely on the enrichment of NARP to synapses in vivo. 1. NARP preferentially localized at synapes 2. NARP mediates physical clustering of neuronal GluRs (using HEK cells as presynaptic surrogates) 3. NARP is in same protein complex as GluRs in vivo 4. Interfering with NARP expression perturbs number of exc. synapses This together makes also the authors conclude "Synaptic Clustering of AMPA Receptors by the Extracellular Immediate-Early Gene Product Narp" (Title). I follow this rationale and propose annotation against term 'neurotransmitter receptor localization to postsynaptic specialization membrane" 22.03.2017 Thomas: above notes were used to update the description except for point 3 as the paper does not show Narp perturbation experiments; they only use a C-terminally truncated NR1 for control transfections (Table 1) that are of no relevance for this annotation. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| Additional literature: | Loss of pentraxin 1 and 2 reduces AMPAR-mediated transmission, likely due to impaired conversion of silent synapses due to reduced AMPA receptor recruitment. These data were obtained in the rodent dorsal lateral geniculate nucleus. @ PMID:20392962 Shows that Astrocyte-Secreted Glypican 4 Regulates Release of Neuronal Pentraxin 1 from Axons to Induce Functional Synapse Formation @ PMID:29024665 |
| SynGO annotation ID: | 312 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |