| Annotated protein: | Neuroplastin (Glycoprotein 55/65) (gp55/65) (Stromal cell-derived receptor 1) (SDR-1). Gene symbol: NPTN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P97546 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NPTN |
| Ontology domain: | Cellular Component |
| SynGO term: | synaptic membrane (GO:0097060) |
| Synapse type(s): | forebrain hippocampus, GABAergic |
| Annotated paper: | Sarto-Jackson I, et al. "The cell adhesion molecule neuroplastin-65 is a novel interaction partner of gamma-aminobutyric acid type A receptors" J Biol Chem. 2012 Apr 20;287(17):14201-14 PMID:22389504 |
| Figure(s): | fig. 1, 3, 4, 5, 6 and 8 |
| Annotation description: | Fig.1 Copurification with GABAA receptor subunit beta2, a known postsynaptic component of gabaergic synapses, from DOC-extracted synaptic forebrain membranes. Fig. 4,5 and 6: Co-localization of neuroplastin and GABAA receptors in dissociated hippocampal neurons in culture using Neuroplastin, GABAA receptor, gephyrin, and VIAAT antibodies (confocal microscopy). Fig. 8: Co-localization of neuroplastin and GABAA receptors with VIAAT in hippocampal DG region (confocal microscopy). Support for postsynaptic localization Fig.3 Direct interaction of NP-65 with GABA-A alpha1 subunit in cis (!) expressed in HEK cells. This means that NP-65 is localized in the same membrane as GABAR subunit --> postsynaptic membrane. 5/12/2016 Pim - Literal Fig 4: " To investigate whether these clusters are found at the synapse, we repeated these experiments (this time in the presence of detergents) by triple staining, including antibodies directed against the VIAAT that supposedly label all GABAergic synapses. As shown in Fig. 4, D1-D4, we find regions with distinct clusters of np, GABA receptor, and VIAAT, indicating that these clusters are most likely synaptically localized." - Literal Fig. 5: " Triple staining indicated the presence of distinct clusters containing np and gephyrin at inhibitory synapses (Fig. 5, A1-A4)." - Literal Fig 6: "The modeled individual synapses (Fig. 6E) revealed a similar topology of the three proteins np, VIAAT, and gephyrin as seen with antibodies that labeled np, VIAAT, and GABAA receptors (Fig. 4E), again suggesting that the three proteins np55/65, gephyrin, and VIAAT were most likely localized at the same synapse." Literal Fig 8: "Fig. 8 shows a distinct area of the dentate gyrus where there was clear co-localization of np65, GABAA receptor β2/3 subunits, and VIAAT (arrows in Fig. 8C4), supporting our co-localization data from hippocampal neurons in culture." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal FRET IP + WB/MSMS |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| SynGO annotation ID: | 31 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |