| Annotated protein: | RNA-binding motif protein, X chromosome (Heterogeneous nuclear ribonucleoprotein G) (hnRNP G) (RNA-binding motif protein, X chromosome retrogene) (RNA-binding motif protein, X chromosome retrogene-like) [Cleaved into: RNA-binding motif protein, X chromosome, N-terminally processed]. Gene symbol: RBMX. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q4V898 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RBMX |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Zhang G, et al. "RNA binding proteins accumulate at the postsynaptic density with synaptic activity" J Neurosci. 2012 Jan 11;32(2):599-609 PMID:22238095 |
| Figure(s): | Table 1, Fig. 5 |
| Annotation description: | Results: -Literal: "Western blots showed that, unlike classical synaptic markers such as SynGAP and Homer1, these hnRNPs were present but not enriched in PSD fractions (Fig. 5A). West- ern blots for postsynaptic (PSD95) and presynaptic (SV2) mark- ers confirmed that we had isolated an enriched PSD fraction (Fig. 5A). To confirm our SILAC results, we performed Western blots for several proteins in PSD fractions isolated from cultured cor- tical neurons stimulated with cLTP BDNF or inhibited with TTX (Fig. 5B). These immunoblots confirmed the SILAC pre- dicted results (Fig. 5B, arrows), showing that hnRNPs M, D, G, and A2/B1 accumulated in PSDs after cLTP BDNF treatment, while SynGAP and homer1 were decreased at PSDs in response to this stimulation (Fig. 5 B, C). No changes in abundance were de- tected for PSD95 or FMRP (FMRP was barely detectable in the PSD fraction; Fig. 5B,C). Using immunocytochemical analysis, we found that hnRNPs G, M, D, and A2/B1 were present in the nucleus (data not shown), as well as in neuronal dendrites and dendritic spines where they colocalized with PSD95 (Fig. 5D). Moreover, using image quantitation, we found that cLTP BDNF resulted in an increase in the colocalization of hnRNPs M, A2/B1, G, and D with PSD-95, which further confirmed our bio- chemical analysis and SILAC results (Fig. 5E)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Western blot Mass-spectrometry Biochemical fractionation (generic) |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 3094 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |