| Annotated protein: | Death-associated protein kinase 1 (DAP kinase 1) (EC 2.7.11.1). Gene symbol: DAPK1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q80YE7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DAPK1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Tu W, et al. "DAPK1 interaction with NMDA receptor NR2B subunits mediates brain damage in stroke" Cell. 2010 Jan 22;140(2):222-34 PMID:20141836 |
| Figure(s): | Fig. 1 |
| Annotation description: | Figure 1 Legend, panels C-G: Literal: " (C and D) The membrane extracts (1 mg proteins) from the cortex of mice 2 hr after sham (lane 1) or MCAO (lane 2) were precipitated with nonspecific IgG (IgG) or anti-NR2B (C) or anti-DAPK1 (D) and probed with anti-DAPK1, anti-NR1, anti-PSD95 or anti-NR2B. Input: 20 mg of protein was loaded in each lane except the PSD95 lane, in which 10 mg proteins were loaded. (E-G) DAPK1+/+ (E) and DAPK1 / (G) cultured neurons were stained with anti-DAPK1 (red) and anti-NR2B (green). A high magnified image (F) from a selected area in (E). In panels A-G, similar results were observed in each of the four experiments." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Microscopy (generic) IP + WB/MSMS |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 3089 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |