| Annotated protein: | Ras-related C3 botulinum toxin substrate 3 (EC 3.6.5.2) (p21-Rac3). Gene symbol: RAC3. Taxonomy: Homo sapiens (Human). Uniprot ID: P60763 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RAC3 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic actin cytoskeleton organization (GO:0098974) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Nestor MW, et al. "The actin binding domain of betaI-spectrin regulates the morphological and functional dynamics of dendritic spines" PLoS One. 2011 Jan 31;6(1):e16197 PMID:21297961 |
| Figure(s): | Fig.4, fig 7b,c & 8 |
| Annotation description: | From Results section: "We first immunoprecipitated endogenous bI-spectrin from cultured cortical neurons and screened for the presence of Rac3 and Rac1. Surprisingly, Rac3 was enriched in immunoprecipitates of bI-spectrin, but Rac1 was absent (3 replicates)(Fig. 8A). We tested the importance of this interaction by co-expressing a dominant negative Rac3 construct together with the ABD of bI-spectrin. Under these conditions, spine head diameters were significantly smaller than in cells expressing the bI-spectrin ABD alone (Fig. 8B,C). Spine head diameters in cells co-expressing the bIspectrin ABD and dominant negative Rac3 or Rac3-DN alone were not significantly different than in cells expressing only GFP, suggesting that Rac3 signaling is required for the effects of the bIspectrin ABD on spines. If so, then this effect should be mimicked by over-expressing wild-type Rac3. Indeed, the size of dendritic spine heads in CA1 pyramidal cells transfected with Rac3 was comparable to spines in cells expressing the ABD of bI-spectrin. Overexpression of Rac3 also resulted in an increase in mEPSC amplitude. The mean amplitude of mEPSCs recorded from neurons expressing Rac3 was not significantly different from those expressing the ABD of bI-spectrin and both were larger than for mEPSCs in untransfected or GFP-transfected cells (Fig. 7B). We conclude that Rac3 can alter dendritic spine structure and function in hippocampal pyramidal cells." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp IP + WB/MSMS |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 3077 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |