| Annotated protein: | Protein Wnt-5a. Gene symbol: WNT5A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9QXQ7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ WNT5A |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic specialization assembly (GO:0099150) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Farias GG, et al. "Wnt-5a/JNK signaling promotes the clustering of PSD-95 in hippocampal neurons" J Biol Chem. 2009 Jun 5;284(23):15857-66 PMID:19332546 |
| Figure(s): | fig 1a,d; 2c,d,e; 3; 4c,d; 5c; 6; 7a,b & s3 |
| Annotation description: | From Results section: "when hippocampal neurons were incubated with the Wnt-5a ligand, an increase in the PSD-95 density was observed (Fig. 1A, a, b, and d, and Table 1) without affecting the clustering of the presynaptic protein synaptophysin (Table 1)." "The co-treatment of Wnt-5a/sFRP prevented the increase of the number of PSD-95 clusters triggered by Wnt-5a (Fig. 1A, b, c, and d)." "Hippocampal neurons exposed to Wnt-5a for 2h presented an 80% increase in the number of PSD-95-containing spines (Fig. 1D, a-g). These results indicate that Wnt-5a but not Wnt-7a affects the postsynaptic region of mammalian synapse by promoting the clustering of PSD-95." "The mean cluster intensity was evaluated to determine whether Wnt-5a decreases the intensity of PSD-95 clusters. Results show that Wnt-5a did not affect the mean intensity of PSD-95 clusters (Fig. 2C), indicating that the increase in the number of PSD-95 clusters is not due to a decrease in the amount of PSD-95 contained in preexistent clusters." "Results show that Wnt-5a did not affect the mean intensity of PSD-95 clusters (Fig. 2C), indicating that the increase in the number of PSD-95 clusters is not due to a decrease in the amount of PSD-95 contained in preexistent clusters." (...) "Results show that Wnt-5a did not affect the mean area of PSD-95 (Fig. 2D) but increased the integrated intensity of PSD-95 (Fig. 2E), indicating that splitting of preexistent PSD-95 clusters did not occur. These results suggest that new clusters of PSD-95 come from a diffuse dendritic cytoplasmic pool without affecting the size or amount of PSD-95 present in preexistent clusters." "We found that hippocampal neurons treated with Wnt-5a for 2 h showed increased levels of PSD-95 in the membrane-enriched fraction with respect to the control (Fig. 3, A and C) (as assessed by the presence of N-cadherin and transferrin receptor and the absence of GAPDH proteins; data not shown) and a concomitant decrease in the PSD-95 levels in the cytoplasmic fraction (Fig. 3, B and D) (as assessed by the presence of β-tubulin and GAPDH and the absence of N-cadherin and transferrin receptor proteins; data not shown)." "Hippocampal neurons incubated with Wnt-5a showed phosphorylation of CAMKII after 5 min, with a peak at 15 min, and then phosphorylation decreased at 30 min and remained at similar levels at 1 h with respect to control conditions (Fig. 4C)." (...) "Wnt-5a increased the phosphorylation of JNK (46-kDa isoform) after 30 min with a peak at 60 h (Fig. 4D), similar to the observed increase in the PSD-95 clustering." (...) "The effect on JNK phosphorylation was antagonized by co-incubation with the Wnt antagonist sFRP at 2 h of treatment (Fig. S3)." "In hippocampal neurons, fumagillin blocked the activation of JNK induced by Wnt-5a and did not affect the β-catenin stabilization (Fig. 5B). Interestingly, fumagillin inhibited the clustering of PSD-95 induced by Wnt-5a at 1 h, without affecting the clustering of PSD-95 (Fig. 5C)" "Hippocampal neurons were incubated with Wnt-5a ligand for 1 h in the presence of KN-93 or BSD-X. However, the Wnt/Ca2+ pathway inhibitors did not affect significantly the PSD-95 clustering induced by Wnt-5a (Fig. 6, A and B. However, when neurons were co-incubated with Wnt-5a in the presence of SP600125, the clustering of PSD-95 induced by Wnt-5a was completely blocked (Fig. 7, A and B). To confirm that the Wnt/JNK pathway is implicated in the clustering of PSD-95, we used anisomycin, a JNK activator (37, 38). Anisomycin induced the clustering of PSD-95 at 1 h of treatment in a way similar to that of Wnt-5a ligand (Fig. 7, A and B)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Western blot |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 3076 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |