| Annotated protein: | Beta-arrestin-2 (Arrestin beta-2). Gene symbol: ARRB2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q91YI4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARRB2 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic signaling pathway (GO:0098926) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Eng AG, et al. "Transduction of group I mGluR-mediated synaptic plasticity by beta-arrestin2 signalling" Nat Commun. 2016 Nov 25;7:13571 PMID:27886171 |
| Figure(s): | Fig.1, 5, 6 |
| Annotation description: | Fig.1: "β-arrestin2 is required for PP-LFS potentiation of mfEPSCs." - "These data demonstrate that PP-LFS, mGluR1-mediated plasticity of mossy fibre-CA3 synapses is dependent specifically on βarr2." Fig.5: "Deficits in CA1 mGluR-LTD in β-arrestin2-/- neurons." - "In contrast, LTD was absent in recordings from βarr2-/- mice (94±10% compared with baseline, n=17, P=0.03, Fig. 5b,c,f). PP-LFS induced equivalent levels of LTD in βarr1+/+ and βarr1-/- mice (78±8% compared with baseline in βarr1+/+, n=17; 70±8% in βarr1-/-, n=12, P=0.39, Fig. 5d-f). Baseline paired pulse facilitation was not different when comparing βarr2-/- and βarr1-/- mice with wild-type littermate groups (1.45±0.05 for βarr2+/+, n=24; 1.38±0.07 for βarr2-/-, n=17, P=0.46; 1.47±0.05 for βarr1+/+, n=17; 1.56±0.11 for βarr1-/-, n=12, P=0.56, Fig. 5g). These results indicate that mGluR-dependent depression of synaptic transmission in CA1 pyramidal neurons is also dependent on βarr2." Fig.6: "mGluR5 is necessary for LTD induced by synaptic stimulation" - "mGluR5 antagonism by MTEP prevented induction of LTD and resulted in equal recovery of EPSC amplitudes measured 25-30 min post-train in βarr2+/+ and βarr2-/- mice (86±4% in wild type, n=14; 96±5% in knockout, n=19; P=0.27, Fig. 6a,b,g)." - "Expression of mGluR5 in total and membrane-enriched fractions also is not grossly affected in βarr2 knockout animals (Supplementary Fig. 4), and thus it is unlikely that the deficit in PP-LFS LTD in βarr2-/- mice can be attributed to loss of surface-expressed mGluR5." Note: beta-arrestins are known regulators of G-protein coupled receptors. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3068 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |