| Annotated protein: | Leucine zipper putative tumor suppressor 3 (ProSAP-interacting protein 1) (ProSAPiP1). Gene symbol: LZTS3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q8K1Q4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LZTS3 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Wendholt D, et al. "ProSAP-interacting protein 1 (ProSAPiP1), a novel protein of the postsynaptic density that links the spine-associated Rap-Gap (SPAR) to the scaffolding protein ProSAP2/Shank3" J Biol Chem. 2006 May 12;281(19):13805-13816 PMID:16522626 |
| Figure(s): | Fig.2, 3, 4 |
| Annotation description: | Fig.2: "Analysis of ProSAPiP1 expression and ProSAP2-ProSAPiP1 interaction" - "Subcellular fractionation aimed to purify protein components of the PSD showed a clear enrichment of the relative amount of ProSAPiP1 immunoreactivity in the PSD fraction as compared with other fractions (Fig. 2F)." Fig.3: " - "Next we determined whether the C terminus of ProSAPiP1 binds in a canonical manner to the PDZ domain of ProSAP2/Shank3 by generating a variety of mutant ProSAPiP1 constructs with point mutations in the last four amino acids, and we tested their binding affinity in a semi-quantitative yeast two-hybrid assay. Inspection of the C terminus of ProSAPiP1 suggests that it should bind to class 1 PDZ domains with the consensus sequence X(T/S)Y(V/L) (34). Accordingly and as reported previously (7), mutations at positions 0 and -2 led to a drastically reduced binding or no binding in the YTH assay. " - "Moreover, pulldown of ProSAP2/Shank3 with a GST-tagged ProSAPiP1 fusion protein from Triton X-100-extracted rat brain homogenates confirmed the results of the co-immunoprecipitation experiments (Fig. 2I)." - "Furthermore, the enrichment of ProSAPiP1 immunoreactivity in PSD fractions was supported by the localization of ProSAPiP1 immunoreactivity at the PSD of excitatory synapses employing immunoelectron microscopy of adult rat brain sections (Fig. 3D). " Fig.4: "ProSAPiP1 localization in cultured hippocampal neurons" - "In 2- and 3-week-old cultures ProSAPiP1 antigen is found in dendrites and spines, although in 4-week-old hippocampal neurons the protein is predominantly localized at synaptic spines. At that time point ProSAPiP1 co-localizes with ProSAP2/Shank3 " |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS Y2H |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3062 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |