| Annotated protein: | Leucine zipper putative tumor suppressor 1 (PSD-Zip70). Gene symbol: LZTS1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q8CFC9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LZTS1 |
| Ontology domain: | Cellular Component |
| SynGO term: | anchored component of postsynaptic density membrane (GO:0099031) |
| Synapse type(s): | hippocampus, glutamatergic neocortex, glutamatergic |
| Annotated paper: | Konno D, et al. "The postsynaptic density and dendritic raft localization of PSD-Zip70, which contains an N-myristoylation sequence and leucine-zipper motifs" J Cell Sci. 2002 Dec 1;115(Pt 23):4695-706 PMID:12415013 |
| Figure(s): | Fig.1, 4, 6 |
| Annotation description: | Fig.1: "Subcellular distribution, predicted amino-acid sequence and N-terminal myristoylation of PSD-Zip70" - "Western blotting of the cerebral subcellular fractions showed that mAb204H specifically recognized a closely related 70 kDa doublet that is concentrated in the PSD-I fraction. A small amount of these 70 kDa proteins was detected in the S2 fraction, which contains the light membrane and the cytosol ( Fig. 1A). mAb204H preabsorbed with GST-Zip70WT (the control) did not detect any proteins in the PSD-I fraction." Fig.4: "Synaptic localization of PSD-Zip70" - "In addition to the patchy fluorescence in the dendrites, intense immunofluorescent clusters of PSD-Zip70 were distributed along the dendrites ( Fig. 4C1,D1 and E1). To further characterize the localization of PSD-Zip70, double staining was performed using pAbZip70 and antibodies against pre- and postsynaptic markers or phalloidin for F-actin. Clusters of PSD-Zip70 were colocalized with PSD-95 clusters ( Fig. 4C1-C3 and C1'-C3') and with many F-actin clusters ( Fig. 4D1-D3 and D1'-D3'). High-resolution images showed that the areas of PSD-Zip70 cluster within the dendritic spines were much wider than those of PSD-95 clusters ( Fig. 4C1'-C3') and that the PSD-Zip70 clusters also partially overlapped with synaptotagmin clusters ( Fig. 4E1-E3 and E1'-E3'), suggesting the synaptic localization of PSD-Zip70." - "Immunogold labeling for PSD-Zip70 was predominantly observed in axospinous and axodendritic asymmetric synapses of pyramidal neurons in the hippocampal CA2 region and cerebral cortex. Most of the immunogold label appeared over the perisynaptic regions of the PSD and the postsynaptic membrane in excitatory asymmetric synapses of pyramidal neurons in the hippocampus and cerebral cortex ( Fig. 4F-I), indicating the postsynaptic localization of PSD-Zip70." Fig.6: "The PSD and raft localization of PSD-Zip70 in the synaptosome fraction" - "As shown in Fig. 6A, PSD-Zip70 and PSD-95 were recovered in both the detergent-insoluble low-density fraction (the raft fraction, fractions 2-4) and the pellet fraction, which probably included the PSD" Note: - "Because PSD-Zip70 is myristoylated on its N-terminus ( Fig. 1D) and also contains the distant polybasic cluster ( Fig. 1B), we examined whether PSD-Zip70's localization to the microvilli may be mediated through N-terminal myristoylation and/or the polybasic cluster. PSD-Zip70N/G2A exclusively accumulated in the nucleus and the perinuclear region ( Fig. 7C1-C3). By contrast, PSD-Zip70C was diffusely distributed throughout the cytoplasm but was not localized to the microvilli and the plasma membrane."..."These results indicate that both N-terminal myristoylation and the polybasic cluster are required for the targeting of PSD-Zip70 to the microvilli and the plasma membrane in epithelial cells." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3060 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |