| Annotated protein: | Ribonuclease 3 (EC 3.1.26.3) (Protein Drosha) (Ribonuclease III) (RNase III). Gene symbol: DROSHA. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q5HZJ0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DROSHA |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Lugli G, et al. "Primary microRNA precursor transcripts are localized at post-synaptic densities in adult mouse forebrain" J Neurochem. 2012 Nov;123(4):459-66 PMID:22897173 |
| Figure(s): | Fig.2, 3 |
| Annotation description: | Fig.2: - " Western blotting of subcellular fractions revealed that both drosha and DGCR8 were present in synaptic fractions and, within synaptosomes, were predominantly associated with PSDs (Fig. 2c)." - "PSD-95 immunoprecipitates contained drosha and DGCR8 (Fig. 2b). " Fig.3: "Immunolocalization of Drosha in adult mouse hippocampus" - " Drosha was colocalized with PSD95 within the neuropil of the molecular layer, where the apical dendrites of pyramidal neurons are located (Fig. 3a and b)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3051 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |