| Annotated protein: | Endoribonuclease Dicer (EC 3.1.26.3) (Double-strand-specific ribonuclease mDCR-1). Gene symbol: DICER1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8R418 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DICER1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | hippocampus, glutamatergic neocortex, glutamatergic |
| Annotated paper: | Lugli G, et al. "Dicer and eIF2c are enriched at postsynaptic densities in adult mouse brain and are modified by neuronal activity in a calpain-dependent manner" J Neurochem. 2005 Aug;94(4):896-905 PMID:16092937 |
| Figure(s): | Fig.3, 6 |
| Annotation description: | Fig.3: "Electron microscopy localization of dicer and eIF2c in dendritic spines in cortex and hippocampal area CA1" - "Electron microscopy immunocytochemistry using peroxidase-based detection confirmed that dicer-like immunoreactivity was present within dendritic spines and appeared to be particularly associated with PSDs (Figs3a and c). eIF2c was also prominent in spines (Fig. 3b)." Fig.6: "Expression of dicer and eIF2c in brain subcellular fractions" - ":A dicer-immunoreactive band was also enriched in PSDs that was approximately twice the size of native dicer, but which was not affected by boiling and reduction of the sample (Fig. 6); it is not clear whether this represents dimeric dicer or a sodium dodecyl sulfate-resistant complex of dicer with another protein(s). " 28/3/2018 Pim - Antibody for Argonaut is not gene specific: "a peptide corresponding to the C-terminal 16 amino acids of eIF2c1 was used as antigen in rabbits (the C-terminal 16 amino acids are identical in eIF2c2; there is one mismatch in eIF2c3 and four mismatches in eIF2c4)." Annotation for this protein was not made. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3049 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |