| Annotated protein: | BAF chromatin remodeling complex subunit BCL11A. Gene symbol: BCL11A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZSY3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ BCL11A |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Kuo TY, et al. "Expression of zinc finger transcription factor Bcl11A/Evi9/CTIP1 in rat brain" J Neurosci Res. 2007 Jun;85(8):1628-36 PMID:17455301 |
| Figure(s): | Fig.4, 5 |
| Annotation description: | Fig.4: "Synaptic distribution of Bcl11A in neurons revealed by immunofluorescence staining" Fig.5: "Subcellular distribution of Bcl11A in rat brain revealed by biochemical fractionation" - "The relative intensity of these protein bands differed among the subcellular compartments, suggesting differential posttranslational modification of Bcl11A-L in different fractions. Immunoblotting of PSD-95 (Fig. 5B) was performed again to ensure the quality of these biochemical fractions (Hsueh and Sheng,1999a). The presence of Bcl11A-S and Bcl11A-L proteins in the crude synaptic membrane and crude synaptic vesicle fractions is consistent with the observation that Bcl11A immunoreactivity was colocalized with synaptophysin in cultured hippocampal neurons, confirming a synaptic distribution of BCl11A. To further elucidate this, the presence of Bcl11A proteins in the postsynaptic density (PSD) fractions was examined. The result showed that Bcl11A-L was enriched in the PSD I and PSD II fractions (Fig. 5C). In contrast, the PSD I and PSD II fractions had only a trace amount of Bcl11A-S (Fig. 5C). This analysis suggested that Bcl11A-L is the major form of postsynaptic Bcl11A protein." 28/3/2018 Pim - The authors claim enrichment in the PSD fraction that I cannot really confirm. I have annotated to the less specific term 'postsynapse'. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3048 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |