Annotated protein:	Kelch-like protein 17 (Actinfilin). Gene symbol: KLHL17. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q8K430
antibody wiki:	search antibodypedia...
SynGO gene info:	SynGO data @ KLHL17
Ontology domain:	Biological Process
SynGO term:	regulation protein catabolic process at postsynapse (GO:0140252)
Synapse type(s):	hippocampus, glutamatergic
Annotated paper:	Salinas GD, et al. "Actinfilin is a Cul3 substrate adaptor, linking GluR6 kainate receptor subunits to the ubiquitin-proteasome pathway" J Biol Chem. 2006 Dec 29;281(52):40164-73 PMID:17062563
Figure(s):	Fig.3, 7
Annotation description:	Title: "Actinfilin Is a Cul3 Substrate Adaptor, Linking GluR6 Kainate Receptor Subunits to the Ubiquitin-Proteasome Pathway" Fig.3: "Actinfilin mediates GluR6 degradation primarily through proteasomes." Fig.7: "Actinfilin and Cul3 negatively regulate surface expression of GluR6" - "We found that decreasing actinfilin levels via RNAi significantly increased surface GluR6 expression in dendritic spines, as assessed morphologically and by increased co-localization with PSD-95 (Fig. 7, A-D and H) and synaptophysin (Fig. 7H). Synaptic GluR6 more than doubled (Fig. 7H), and the size of the GluR6-containing puncta increased 1.8-fold (mean punctal area ± S.E.: control neurons, 0.12 ± 0.01 μm2; RNAi-AF expressing neurons, 0.22 ± 0.01 μm2). "
Evidence tracking, Biological System:	Cultured neurons Non-neuronal tissue
Evidence tracking, Protein Targeting:	RNAi / shRNA Over-expression
Evidence tracking, Experiment Assay:	Confocal
Annotator(s):	Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:	Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:	3039
Dataset release (version):	20231201
View annotation as GO-CAM model: