| Annotated protein: | Kelch-like protein 17 (Actinfilin). Gene symbol: KLHL17. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q8K430 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KLHL17 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic cerebellum, glutamatergic |
| Annotated paper: | Chen Y, et al. "Actinfilin, a brain-specific actin-binding protein in postsynaptic density" J Biol Chem. 2002 Aug 23;277(34):30495-501 PMID:12063253 |
| Figure(s): | Fig.5, 7 |
| Annotation description: | Fig.5: "Actinfilin in the postsynaptic density (PSD) preparation" - "Actinfilin also had the same separation profile as PSD95 before the Triton X-100 treatment (Fig. 5,lanes 1-9) and was abundant in the synaptosome fraction (Fig. 5, lane 8). After Triton X-100 treatment, most of the actinfilin, however, was released from the PSD pellet by the detergent treatment that led to only a small fraction of actinfilin presented in the PSD preparation (Fig. 5, lanes 9-13). This result suggests that actinfilin was not the core component of the PSD protein complex." Fig.7: "Immunogold labeling for actinfilin in the cerebellum (A and B) and the hippocampus (C and D). " - " In the cerebellum (Fig.7, A and B) and the hippocampus (Fig. 7, C and D), immunogold labeling for actinfilin was associated most commonly with dendrite cytoplasm. Often the labeling decorated membranous structures on the cytoplasmic side of the plasmic membrane or within the cytoplasm. Substantial labeling was seen in the dendrites of pyramidal cells of the hippocampus and Purkinje cells (Fig. 7 B) of the cerebellum, as well as in interneuron dendrites (Fig. 7 D). Labeling of synapses was variable. In many cases, a moderate postsynaptic labeling was observed, with gold particles associated with membranous cytoplasmic structures as in the dendrites (Fig. 7,B and D), as well as with the postsynaptic density and postsynaptic side of membrane (Fig. 7, C andD)." Note: although localization to the PSD is shown, I did not annotate to this term. This is caused by the interaction of Actinfilin with F-actin and the rather weak interaction with the PSD as shown in Fig.5. I think a term such as 'postsynaptic actin cytoskeleton' would be most acurate, but since the data do not really support this, I have not used this term. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3038 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |