| Annotated protein: | Huntingtin interacting protein 1 related. Gene symbol: HIP1R. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q99PW9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HIP1R |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Okano A, et al. "Huntingtin-interacting protein-1-related protein of rat (rHIP1R) is localized in the postsynaptic regions" Brain Res. 2003 Mar 28;967(1-2):210-25 PMID:12650982 |
| Figure(s): | Fig.7, 9 |
| Annotation description: | Fig.7: "Subcellular distribution of rHIP1R protein in the rat brain and comparison with that of huntingtin." - "The rHIP1R immunoreactive protein of 120 kDa was detected in the total homogenate, P1 (cell debris and nuclei), the synaptosome and the SPM fractions. The rHIP1R-immunoreactive protein was enriched in the SPM fraction and a faint band was detected in the synaptosome fraction, but was not present in the PSD fraction that was obtained after Triton X-100 treatment and Triton X-100-KCl washing of SPM. " Fig.9: "Electron microscopic examination of rHIP1R in the rat cerebral cortex" - "Immunoreactivities were observed in the cytoplasmic structures in the spines including PSDs and structures which were attached or very close to PSD and apart from PSD (Fig. 9)." - "The presynaptic terminals were constantly negative for staining." Note: the patches in the postsynapse may be the endocytic zone (see PMID:11564758) but this is not demonstrated. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Huntingtin-interacting protein-1-related protein; HIP1R is a adaptor protein for clathrin and is involved in endocytosis. @ PMID:11564758 |
| SynGO annotation ID: | 3033 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |