| Annotated protein: | Double-stranded RNA-binding protein Staufen homolog 2 (r-staufen protein). Gene symbol: STAU2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q68SB1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STAU2 |
| Ontology domain: | Biological Process |
| SynGO term: | anterograde dendritic transport of messenger ribonucleoprotein complex (GO:0098964) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Goetze B, et al. "The brain-specific double-stranded RNA-binding protein Staufen2 is required for dendritic spine morphogenesis" J Cell Biol. 2006 Jan 16;172(2):221-31 PMID:16418534 |
| Figure(s): | Fig.1, 2, 3, 4 |
| Annotation description: | Fig.1: "The intron-containing Calm3 mRNA isoform interacts with Stau2 and localizes to dendrites" - "This was confirmed by IP experiments: Only Calm3 transcripts were highly enriched in the immunoprecipitates of Stau2-containing RNPs (Fig 1B) [10], but no enrichment was obtained with control PIS IPs." Fig.2: "Neuronal activity regulates dendritic localization of Calm3 intron-containing endogenous and GFP reporter RNAs" - Note: NMDA is used for stimulation which provides synaptic context to this data. Fig.3: "Stau2 overexpression increases the dendritic localization of Calm3 intron-containing GFP reporter mRNA" - "To investigate whether Stau2 directly mediates dendritic localization of intron-containing transcripts, we evaluated the subcellular localization of the different GFP mRNA reporters when they were co-expressed with the exogenous 62-kDa isoform of Stau2 (TagRFP-Stau262). TagRFP-Stau262 expression significantly increased the dendritic localization of the intron-containing reporter mRNAs (Figs 3A and B, and EV3A and B). " Fig.4: "Dendritic localization of endogenous intron-containing Calm3L mRNA is regulated by Stau2 expression levels" - "Here, we observed that downregulation of Stau2 using transiently transfected shRNA (shStau2) [21] in rat hippocampal neurons led to substantial reduction of dendritically localized intron-retaining Calm3L transcripts (Fig 4A and C), while a control shRNA (shControl) did not (Fig EV4A). Importantly, the reduction in dendritic localization of Calm3L mRNA was completely rescued when an RNAi-resistant Stau2 (Stau2R) [6] was co-expressed together with shStau2 (Fig 4B and C). " Note: a combination of Stau2; Staufen 2 perturbation and NMDA application should have been included in the experiments. I do not know why this is not included in the paper. The authors mention: " Since this dendritic Calm3L mRNA localization is regulated by NMDA receptor activation, it is tempting to speculate that Calm3L recruitment enables local protein synthesis at synapses." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | IP + DNA sequencing Fluorescence in situ hybridization (FISH) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3029 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |