| Annotated protein: | Vesicle-associated membrane protein 4. Gene symbol: VAMP4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A096MJ99 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VAMP4 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle to endosome fusion (GO:0016189) |
| Synapse type(s): | brain |
| Annotated paper: | Rizzoli SO, et al. "Evidence for early endosome-like fusion of recently endocytosed synaptic vesicles" Traffic. 2006 Sep;7(9):1163-76 PMID:17004320 |
| Figure(s): | Fig. 5 |
| Annotation description: | In this study, Rizzoli et al. showed that synaptosomal endocytic vesicles have the ability to fuse to PC12-cell acceptor endosomes. And the fusion predominantly depends on endosomal SNAREs, such as Syntaxin 6 and Vamp4. In order to show that this fusion is specifically mediated by endosomal SNARES and not exocytotic SNAREs, they added recombinant SNARE proteins to the fusion reactions. The result revealed that synaptobrevin 2 and combination of SNAP 25/syntaxin does not block the fusion; however, VAMP4 and a combination of vti1a, syntaxin 6 and syntaxin 13 significantly inhibited the reaction. |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Optical physiology |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | In this study, Zwilling et al. showed that SNAREs mediating homotypic fusion of early endosomes (containing Vamp4) fuse to liposomes. @ PMID:17159904 In this paper, it was shown that VAMP4 could selectively maintain bulk Ca2+-dependent asynchronous release and some spontaneous release. Also, the interaction of Vamp4 with syntaxin1 and SNAP-25 was documented. However, this complex is not able to bind to syt1 and complexin that are required for fast synchronous neurotransmitter release. @ PMID:22406549 |
| SynGO annotation ID: | 302 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |