Annotated protein:	Catechol O-methyltransferase (EC 2.1.1.6). Gene symbol: COMT. Taxonomy: Mus musculus (Mouse). Uniprot ID: O88587
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SynGO gene info:	SynGO data @ COMT
Ontology domain:	Biological Process
SynGO term:	neurotransmitter catabolic process (GO:0042135)
Synapse type(s):	neocortex
Annotated paper:	Kaenmaki M, et al. "Quantitative role of COMT in dopamine clearance in the prefrontal cortex of freely moving mice" J Neurochem. 2010 Sep;114(6):1745-55 PMID:20626558
Figure(s):	Fig.3, 4, 5, 6
Annotation description:	Note: COMT metabolizes dopamine to an inactive compound (3-methoxytyramine). Abstract: "Here, we employ two types of microdialysis to study these pathways using DAT, NET and MAO blockers in conscious mice, with or without Comt gene disruption. In quantitative no-net-flux microdialysis, DA levels were increased by 60% in the PFC of COMT-knockout (ko) mice, but not in the striatum and nucleus accumbens. In conventional microdialysis studies, we showed that selective NET and MAO inhibition increased DA levels in the PFC of wild-type mice by two- to fourfold, an effect that was still doubled in COMT-ko mice. Inhibition of DAT had no effect on DA levels in either genotype. Therefore, we conclude that in the mouse, PFC COMT contributes about one half of the total DA clearance."
Evidence tracking, Biological System:	Intact tissue
Evidence tracking, Protein Targeting:	Genetic transformation (eg; knockout, knockin, mutations)
Evidence tracking, Experiment Assay:	Voltammetry assay
Annotator(s):	Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:	Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:	3011
Dataset release (version):	20231201
View annotation as GO-CAM model: