| Annotated protein: | Drebrin-like protein (Actin-binding protein 1) (Abp1) (SH3 domain-containing protein 7). Gene symbol: DBNL. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9JHL4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DBNL |
| Ontology domain: | Biological Process |
| SynGO term: | structural constituent of postsynaptic actin cytoskeleton (GO:0098973) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Haeckel A, et al. "The actin-binding protein Abp1 controls dendritic spine morphology and is important for spine head and synapse formation" J Neurosci. 2008 Oct 1;28(40):10031-44 PMID:18829961 |
| Figure(s): | Fig.1, 2, 3, 4, 5, 7 |
| Annotation description: | Fig.1: "Expression of Abp1 full length and fragments thereof containing the F-actin binding domains induce increased spine length" Fig.2: "Abp1 overexpression results predominantly in elongation of mushroom-type spines" Fig.3: "Effects of full-length Abp1 and actin interaction domains of Abp1 on the density of mushroom-shaped spines and synapses" Fig.4: "Overexpression of Abp1 fragments encompassing the C-terminal SH3 domain highly significantly decreases the density of mushroom spines" Fig.5: "RNAi-based reduction of Abp1 expression levels reduces the densities of mushroom spines and synapses on hippocampal neurons" Fig.7: "Abp1 and ProSAP1 or ProSAP2 coexpression leads to highly branched and complex spines with multiple enlarged spine heads" Discussion: ". Abp1 is not only able to link dynamic actin fibers to ProSAP/Shank proteins and to stabilize actin filaments (Fig. 12A,B), but it is also able to link the SH3 domain interaction partners of the WASP superfamily of Arp2/3 complex activators with F-actin and to activate N-WASP. This leads to Abp1-controlled Arp2/3 complex-mediated nucleation of actin filaments in the microenvironment of the actin- and ProSAP/Shank-rich PSD. Formation of new actin filaments then provides even more Abp1 attachment sides, because Abp1 prefers freshly polymerized actin filaments (Kessels et al., 2000). " |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3006 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |