| Annotated protein: | Serine/threonine-protein kinase PAK 3 (EC 2.7.11.1) (Beta-PAK) (CDC42/RAC effector kinase PAK-B) (p21-activated kinase 3) (PAK-3). Gene symbol: PAK3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61036 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PAK3 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse organization (GO:0099175) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Jaudon F, et al. "The RhoGEF DOCK10 is essential for dendritic spine morphogenesis" Mol Biol Cell. 2015 Jun 1;26(11):2112-27 PMID:25851601 |
| Figure(s): | Fig.7 |
| Annotation description: | Fig.7: "DOCK10 regulates spine formation via Cdc42-N-WASP and -PAK3 pathways" - "As shown previously, expression of N-WASPΔWA, a construct encoding N-WASP deleted of its C-terminal WA domain and thus unable to bind the Arp2/3 complex and G-actin (Supplemental Figure S4; Wegner et al., 2008 blue right-pointing triangle), or expression of this domain alone (N-WASP WA) affected the number of spines in cultured hippocampal neurons (Figure 7C). Expression of a kinase-dead form of PAK3 (PAK3 KD; Supplemental Figure S4) also affected spine number (Figure 7C). Strikingly, coexpression of DOCK10 with either N-WASPΔWA or N-WASP WA or PAK3 KD impaired DOCK10's effect on spine formation (Figure 7C)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 3003 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |