Annotated protein:Actin nucleation-promoting factor WASL (Neural Wiskott-Aldrich syndrome protein) (N-WASP). Gene symbol: WASL. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q91YD9
antibody wiki:
SynGO gene info:SynGO data @ WASL
Ontology domain:Biological Process
SynGO term:regulation of postsynapse organization (GO:0099175)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Jaudon F, et al. "The RhoGEF DOCK10 is essential for dendritic spine morphogenesis" Mol Biol Cell. 2015 Jun 1;26(11):2112-27 PMID:25851601
Figure(s):Fig.7
Annotation description:Fig.7: "DOCK10 regulates spine formation via Cdc42-N-WASP and -PAK3 pathways"
- "As shown previously, expression of N-WASPΔWA, a construct encoding N-WASP deleted of its C-terminal WA domain and thus unable to bind the Arp2/3 complex and G-actin (Supplemental Figure S4; Wegner et al., 2008 blue right-pointing triangle), or expression of this domain alone (N-WASP WA) affected the number of spines in cultured hippocampal neurons (Figure 7C). Expression of a kinase-dead form of PAK3 (PAK3 KD; Supplemental Figure S4) also affected spine number (Figure 7C). Strikingly, coexpression of DOCK10 with either N-WASPΔWA or N-WASP WA or PAK3 KD impaired DOCK10's effect on spine formation (Figure 7C)."
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Over-expression
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Pim van Nierop (ORCID:0000-0003-0593-3443)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:3002
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology