| Annotated protein: | Elongation factor 2 (EF-2) (EC 3.6.5.-). Gene symbol: EEF2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P05197 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EEF2 |
| Ontology domain: | Biological Process |
| SynGO term: | translation at postsynapse (GO:0140242) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Verpelli C, et al. "Synaptic activity controls dendritic spine morphology by modulating eEF2-dependent BDNF synthesis" J Neurosci. 2010 Apr 28;30(17):5830-42 PMID:20427644 |
| Figure(s): | Fig.2, 3, 4, 5, 6, 7, 8 |
| Annotation description: | Note: the role of eEF2 is not shown via perturbation in these experiments. The annotation rest on 1] the known role of eEF2 in protein translation, 2] the established modulatory role of eEF2 Thr56 phosporylation ("eEF2 phosphorylation at Thr56 by the specific eEF2 kinase (eEF2K) usually slows protein translation and elongation (Ryazanov et al., 1988; Ryazanov et al., 1991) but increases the translation of some mRNAs localized in dendrites including αCaMKII and Arc (Marin et al., 1997; Scheetz et al., 2000; Chotiner et al., 2003; Belelovsky et al., 2005; Park et al., 2008)."), and the eEF2K-mediated pathway demonstrated in the experiments to regulate protein translation. Fig.2: "eEF2 phosphorylation by eEF2K plays a key role in remodeling spine morphology" Fig.3: "eEF2 phosphorylation by eEF2K regulates synapse morphology" Fig.4: "Activity regulates eEF2K-dependent spine morphogenesis" Fig.5: "eEF2 phosphorylation by eEF2K regulates local protein synthesis" Fig.6: "Neuronal activity modulates BDNF protein and transcripts in a biphasic manner, through the eEF2-eEF2K pathway" Fig.7: "Activity modulates BDNF synthesis in dendrites" Fig.8: "BDFN overexpression rescues spine phenotype due to eEF2K knock-down" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2993 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |