| Annotated protein: | Dapper homolog 1 (Dapper antagonist of catenin 1) (Frodo homolog) (MDpr1) (Thymus-expressed novel gene 3 protein). Gene symbol: DACT1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8R4A3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DACT1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Okerlund ND, et al. "Dact1 is a postsynaptic protein required for dendrite, spine, and excitatory synapse development in the mouse forebrain" J Neurosci. 2010 Mar 24;30(12):4362-8 PMID:20335472 |
| Figure(s): | Fig.1 |
| Annotation description: | Fig.1: "Dact1 is present at forebrain synapses" - "This staining, which was absent from Dact1-/- HCNs (Fig 1B), colocalized with synaptic markers, including at punctate intensities of the excitatory postsynaptic marker protein PSD95 likely to correspond to postsynaptic sites (Fig 1C-H). Supporting a postsynaptic localization for Dact1, immunoblotting of subcellular fractions derived from forebrain cortical cultures revealed that recombinantly expressed FLAG-Dact1 co-partitioned with PSD95 in postsynaptic, as opposed to presynaptic, fractions (Fig 1I)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2987 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |