| Annotated protein: | Ras-related protein Rap-1b (EC 3.6.5.2) (GTP-binding protein smg p21B). Gene symbol: RAP1B. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q99JI6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RAP1B |
| Ontology domain: | Biological Process |
| SynGO term: | modification of postsynaptic structure (GO:0099010) |
| Synapse type(s): | basal ganglia, glutamatergic |
| Annotated paper: | Cahill ME, et al. "Bidirectional Synaptic Structural Plasticity after Chronic Cocaine Administration Occurs through Rap1 Small GTPase Signaling" Neuron. 2016 Feb 3;89(3):566-82 PMID:26844834 |
| Figure(s): | fig 4c-k, 5 |
| Annotation description: | From Results section: "Thin spines are typically considered immature, while stubby and mushroom spines are typically considered mature (Nimchinsky et al., 2002). We overexpessed HSV-Rap1b-GFP or HSV-DN-Akt-GFP into NAc either alone or together for 3 days (Figures 4B and 4C). Rap1b overexpression increased the total density of dendritic spines in NAc MSNs, an effect blocked by concurrent overexpression of DN-Akt; 3-day DN-Akt overexpression by itself did not affect total spine density (Figure 4D). Of the spine subtypes, Rap1b induced a selective increase in the density of thin spines in NAc, an effect blocked by concomitant overexpression of DN-Akt (Figures 4E-4G)." "First, we found that Rap1b overexpression alone, but not in combination with DN-Akt, reduced mean spine head diameter across all spine classes (Figure 4H). To determine if this reflects changes within particular spine types, we analyzed cumulative plots of spine head diameter separately for thin, stubby, and mushroom spines and found that Rap1b overexpression produced a leftward shift in the cumulative curve of both mushroom and stubby spines, indicative of a reduction in head diameter for these spine types. No significant change in the cumulative curve of thin spines was detected (Figures 4I-4K). Collectively, these spine data indicate that the reduction in mean spine head diameter upon Rap1b overexpression in NAc MSNs stems from both an increase in thin spine density and a reduction in the head diameter of more mature spines." From Discussion: "We found that short-term disruption of Akt activity inhibits Rap1b's ability to induce de novo thin spine formation in NAc MSNs, indicating that Akt is a key mediator of Rap1b's effects on synaptic structural plasticity in NAc." 9/4/2018 Pim Fig.5: "Rap1b and Akt are Necessary for Cocaine-Induced Spine Remodeling in NAc MSNs" |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 2985 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |