| Annotated protein: | Voltage-dependent anion-selective channel protein 1 (VDAC-1) (rVDAC1) (Outer mitochondrial membrane protein porin 1). Gene symbol: VDAC1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z2L0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VDAC1 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic active zone membrane (GO:0048787) |
| Synapse type(s): | brain |
| Annotated paper: | Morciano M, et al. "The proteome of the presynaptic active zone: from docked synaptic vesicles to adhesion molecules and maxi-channels" J Neurochem. 2009 Feb;108(3):662-75 PMID:19187093 |
| Figure(s): | fig 1, 3, table 1 |
| Annotation description: | From the Results section: "We further characterized the proteins contained in the immunoisolated active zone by western blot analysis following 2-dimensional BAC/SDS-PAGE." (...) "Other proteins identified included (...) and the ion channel VDAC1." "Proteins of the presynaptic active zone were separated by SDS-PAGE and 1 mm gel slices were trypsinized and subjected to nanoLC ESI MS/MS." (...) "Combining the nanoLC ESI MS/MS data with those obtained by western blotting and MALDI-TOF-MS (Table S2) we identified a total of 240 different proteins (Table 1)." 27/3/2018 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Mass-spectrometry |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 2982 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |