| Annotated protein: | Serine/threonine-protein kinase TAO2 (EC 2.7.11.1) (Thousand and one amino acid protein 2). Gene symbol: TAOK2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9JLS3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TAOK2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse organization (GO:0099175) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Yadav S, et al. "TAOK2 Kinase Mediates PSD95 Stability and Dendritic Spine Maturation through Septin7 Phosphorylation" Neuron. 2017 Jan 18;93(2):379-393 PMID:28065648 |
| Figure(s): | Fig.8 |
| Annotation description: | Fig.8: "Phosphorylated Sept7 Interacts with PSD95 and Restricts Its Mobility" - "To test whether the stable spine-associated population of Sept7 could associate with PSD95 and restrict its mobility, thereby mediating spine stability and its maturation, we measured the effect of TAOK2 mediated Sept7 phosphorylation on the mobility of PSD95 in dissociated neurons. Neurons were transfected with GFP-tagged PSD95 along with either control shRNA, TAOK2 specific shRNA, phosphomutant Sept7T426A, or phosphomimetic Sept7T426D. Individual PSD95-GFP punctae in each condition were then photobleached, and the extent of fluorescence recovery was measured over a period of 20 min (Figures 8B-8D). The mobile fraction of PSD95 increased from 45.75% in control to 77.76% in TAOK2 knockdown neurons. Likewise, approximately 77% of PSD95 was in the mobile fraction in neurons expressing the phosphomutant Sept7T426A. In contrast, expression of the phosphomimetic Sept7T426D further reduced the mobile fraction of PSD95 to 36.5% of the total population, as compared to the 45% in control neurons (Figure 8D). These data indicate that the mobility of PSD95 is regulated by TAOK2 mediated phosphorylation of Sept7, providing a direct mechanism for dendritic spine stability." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2980 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |