| Annotated protein: | Septin-7 (CDC10 protein homolog). Gene symbol: SEPTIN7. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9WVC0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SEPTIN7 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic septin cytoskeleton (GO:0150050) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Yadav S, et al. "TAOK2 Kinase Mediates PSD95 Stability and Dendritic Spine Maturation through Septin7 Phosphorylation" Neuron. 2017 Jan 18;93(2):379-393 PMID:28065648 |
| Figure(s): | Fig.7, 8 |
| Annotation description: | Fig.7: "Sept7 Localization and Mobility Is Regulated by TAOK2 Mediated Phosphorylation" - "We next tested whether phosphorylation of Sept7 at T426 changed its dynamics of assembly. By measuring the fluorescence recovery after photobleaching, we found that GFP-tagged Sept7 T426D enriched at the spines was more stable than the phosphomutant Sept7 T426A that accumulated at the base of the dendritic spine." - "In neurons transfected with phosphomimetic or wild-type Sept7 along with the synaptic scaffolding protein PSD95, we observed much greater co-localization of Sept7T426D with PSD95 in the spine head as compared to Sept7WT (Figures 7G and 7H). " Fig.8: "Phosphorylated Sept7 Interacts with PSD95 and Restricts Its Mobility" - "Using the pSept7 (T426) antibody, we found that phosphorylated Sept7 co-immunoprecipitated with PSD95 from mouse brain lysates, indicating that phosphorylated Sept7 and PSD95 interact in vivo (Figure S7E)" - "Moreover, endogenous PSD95 from the brain lysate was pulled down by Sept7-CWT that was incubated with wild-type TAOK2, but not the kinase-dead mutant, whereas mutation of T426 to alanine completely abolished the interaction between Sept7 C-terminal tail and PSD95(Figures 8A,S7F, and S7G)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2978 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |