| Annotated protein: | Endophilin-B2 (SH3 domain-containing GRB2-like protein B2). Gene symbol: SH3GLB2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q5PPJ9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SH3GLB2 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic neurotransmitter receptor endocytosis (GO:0098884) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Loebrich S, et al. "CPG2 Recruits Endophilin B2 to the Cytoskeleton for Activity-Dependent Endocytosis of Synaptic Glutamate Receptors" Curr Biol. 2016 Feb 8;26(3):296-308 PMID:26776730 |
| Figure(s): | Fig.5, 6 |
| Annotation description: | Fig.5: "CPG2 and EndoB2 Co-localize and Recruit Components of the Endocytic Machinery to F-Actin" - "Triton X-100 extracted neurons were infected with lentivirus expressing a control construct or the CPG2 knockdown construct [7, 8]. The intensity of clathrin heavy chain immunofluorescence in dendritic spines was significantly reduced when CPG2 was knocked down as compared to control (Figures 5C, left, and 5D)." - "Similar to the effect of knocking down CPG2, knockdown of EndoB2 using hp62 significantly reduced clathrin heavy chain association with the spine cytoskeleton after Triton X-100 extraction (Figures 5C, right, and 5D). " Fig.6: "EndoB2 Is Required for Activity-Dependent, but Not Constitutive, Internalization of AMPA- and NMDA-Type Glutamate Receptors" - "Constitutive internalization of AMPA- and NMDA-type glutamate receptors seemed unaffected by EndoB2 KD (Figures 6A and 6B)." - "We found a similar result, an almost 3-fold increase in GluA1 internalization in response to picrotoxin (Figure S2). Surprisingly, EndoB2 knockdown resulted in a significant decrease in activity-dependent internalization not only of GluA1 glutamate receptor subunits but also of GluA2 and GluN1 subunits (Figures 6C and 6D)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Western blot |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2977 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |