| Annotated protein: | Palmitoyltransferase ZDHHC5 (EC 2.3.1.225) (Zinc finger DHHC domain-containing protein 5) (DHHC-5). Gene symbol: ZDHHC5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q2THW7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ZDHHC5 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Brigidi GS, et al. "Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5" Nat Commun. 2015 Sep 3;6:8200 PMID:26334723 |
| Figure(s): | Fig.9 |
| Annotation description: | Fig.8: "Activity-induced AMPAR surface insertion requires DHHC5 endocytic cycling" - "Neurons expressing DHHC5 shRNA exhibited a significant reduction in the IntDen of SEP-GluA1 under basal conditions (Fig. 9a,b), in agreement with the requirement of DHHC5 for Grip1b palmitoylation and constitutive synaptic delivery of AMPARs11" - "The IntDen of SEP-GluA1 was significantly increased 20 min after cLTP in shRNA-c control cells, relative to the same clusters before stimulation (Fig. 9a,b), consistent with previous reports12,22,28,33. This activity-induced increase in SEP-GluA1 clusters was abolished in shRNA-expressing neurons (Fig. 9a,b), in agreement with the requirement for δ-catenin palmitoylation in activity-induced surface localization of AMPARs12. DHHC5 WT*, but not Y533E*, rescued the knockdown phenotype (Fig. 9a,b), indicating the requirement of DHHC5 internalization for the activity-mediated stabilization of AMPAR at the synapse. " |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | See also this reference on Grip1b palmitoylation by DHHC5. @ PMID:22325201 |
| SynGO annotation ID: | 2974 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |