| Annotated protein: | Glutamate receptor-interacting protein 2 (GRIP-2) (AMPA receptor-interacting protein GRIP2). Gene symbol: GRIP2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9WTW1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRIP2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | neocortex, glutamatergic hippocampus, glutamatergic |
| Annotated paper: | Srivastava S, et al. "Novel anchorage of GluR2/3 to the postsynaptic density by the AMPA receptor-binding protein ABP" Neuron. 1998 Sep;21(3):581-91 PMID:9768844 |
| Figure(s): | Fig.4 |
| Annotation description: | Fig.4: "ABP Is Located at Synaptic Junctions" - "To establish the synaptic colocalization of ABP, the distribution of ABP was examined by electron microscopy of immunogold-labeled sections of adult rat brain. This confirmed that ABP is present at the postsynaptic membrane of excitatory synapses (Figure 4B). In blocks from layers II-III of somatic sensory cortex and from stratum radiatum of hippocampal CA2, gold particles coding for ABP showed a clear association with asymmetric synapses, though not all synapses were labeled." - " Labeling was at its highest concentration 15-30 nm inside the postsynaptic membrane, extending ~100 nm into the cytoplasm (Figure 4C). Examining labeling on the "lateral" axis (tangential to the synaptic membrane), particles within 40 nm of the postsynaptic membrane were almost completely confined to the synaptic active zone (Figure 4D)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2966 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |