| Annotated protein: | F-box only protein 2. Gene symbol: FBXO2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q80UW2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ FBXO2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of protein catabolic process at postsynapse, modulating synaptic transmission (GO:0099576) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Kato A, et al. "Activity-dependent NMDA receptor degradation mediated by retrotranslocation and ubiquitination" Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5600-5 PMID:15809437 |
| Figure(s): | Fig.4 |
| Annotation description: | Fig.4: "Activity-dependent degradation of NR1 by Fbx2" - "To evaluate possible roles for Fbx2 in this process, we overexpressed GFP-tagged Fbx2(WT) or Fbx2(ΔF) in hippocampal cultured neurons and recorded NMDA-receptor-mediated currents in outside-out somatic membrane patches (Fig. 4 a and b). These patches contain extrasynaptic NMDA receptors." - "We found a 2-fold increase in NMDA-receptor-mediated responses in neurons expressing Fbx2(ΔF) that were treated with the GABAA-receptor antagonist BIC (30.6 ± 5.3 pA, n = 16 vs. 14.3 ± 0.6 pA, n = 7 in uninfected cells). This effect was activity-dependent, because Fbx2(ΔF) overexpression did not affect NMDA currents under control conditions or in cultures treated with TTX (control, 19.8 ± 2.9 pA, n = 15; TTX, 13.2 ± 2.1 pA, n = 14). Overexpression of Fbx2(WT) did not affect NMDA currents (control, 13.5 ± 2.6 pA, n = 5; BIC, 13.4 ± 2.4 pA, n = 7; TTX, 12.8 ± 2.0 pA, n = 7). Paralleling these electrophysiological changes, we found that Fbx2(ΔF) dramatically increased NR1 immunoreactivity in somatic regions of neurons treated with BIC (256 ± 24%) (Fig. 4c). We also detected activity-dependent ubiquitination of NR1 (Fig. 4d)." |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Protein-protein interaction (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2965 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |