| Annotated protein: | Neuronal pentraxin-2 (NP2) (Neuronal activity-regulated pentraxin) (Neuronal pentraxin II) (NP-II). Gene symbol: NPTX2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P97738 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NPTX2 |
| Ontology domain: | Cellular Component |
| SynGO term: | synaptic cleft (GO:0043083) |
| Synapse type(s): | spinal cord, glutamatergic hippocampus, glutamatergic |
| Annotated paper: | O'Brien RJ, et al. "Synaptic clustering of AMPA receptors by the extracellular immediate-early gene product Narp" Neuron. 1999 Jun;23(2):309-23 PMID:10399937 |
| Figure(s): | Fig 6 |
| Annotation description: | Narp, a secreted protein, accumulates at the surface of excitatory synapses on live labelled cultured neurons (Fig. 3). In immuno-EM, Narp1 labeling is predominant within the synaptic cleft (Fig. 6). Its localization to the postsynaptic specialization membrane is in agreement with the interaction of Narp with the extracellular domains of heterologously expressed GluA1/2/3 and its formation of a complex with GluA1 in the brain (Fig. 7, 8). 19/3/2018 Pim - Fig.6: "Gold particles were also frequently observed in the synaptic cleft." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal IP + WB/MSMS |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| Additional literature: | This immunohistochemical study supports that Narp1 is preferentially targeted to axonal projections in several neuronal populations, including hippocampal granule cells and cortical neurons. This implies that it is released from presynaptic terminals to bind postsynaptic AMPA receptors. @ PMID:12358799 |
| SynGO annotation ID: | 2963 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |