| Annotated protein: | Endophilin-A1 (Endophilin-1) (SH3 domain protein 2A) (SH3 domain-containing GRB2-like protein 2) (SH3p4). Gene symbol: SH3GL2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q62420 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SH3GL2 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic actin cytoskeleton organization (GO:0098974) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Yang Y, et al. "Endophilin A1 regulates dendritic spine morphogenesis and stability through interaction with p140Cap" Cell Res. 2015 Apr;25(4):496-516 PMID:25771685 |
| Figure(s): | Fig.7, 8 |
| Annotation description: | Fig.7: "Endophilin A1 regulates spine morphogenesis via modulating actin cytoskeleton reorganization" - "Quantitative results showed that there was about 2-fold decrease in the intensity of LifeAct-mCherry in spines of neurons silenced for endophilin A1 or p140Cap than that in control (Figure 7B), indicating a decrease in F-actin enrichment in spines by downregulation of the endophilin A1-p140Cap pathway. Moreover, immunofluorescence staining showed that knockdown of endophilin A1 also caused a decrease in the intensity of p140Cap signals in spines (Figure 7C and and7D).7D). Consistently, immunoblotting analysis detected a decrease in p140Cap protein levels in the PSD fraction of endophilin A1-silenced neurons (Figure 7E and and7F).7F). Moreover, knockdown of endophilin A1 or p140Cap reduced the protein levels of cortactin and PSD95 but not drebrin, another cytoskeleton regulator, in the PSD fraction (Figure 7E, 7G-7J). . Together, these data indicate that endophilin A1 regulates the distribution of both p140Cap and cortactin in dendritic spines to modulate enrichment of F-actin during spine morphogenesis." Fig.8: "Endophilin A1-p140Cap regulates spine morphogenesis, synapse formation and synaptic function through cortactin" - "we analyzed spine morphology and synapse formation of cultured hippocampal neurons co-transfected with endophilin A1-shRNA and Flag-tagged cortactin expression constructs. The defects in spine morphology and synapse formation indicated by PSD95 or SYP puncta on dendrites of endophilin A1-silenced neurons were fully rescued by overexpression of cortactin (Figure 8A-8H). Furthermore, the decreased frequency of mEPSCs induced by endophilin A1 knockdown was also rescued by overexpression of p140Cap or cortactin (Figure 8I-8K), indicating that endophilin A1-p140Cap regulates reorganization of actin cytoskeleton through cortactin in spine morphogenesis, synapse formation and synaptic function." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2960 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |