| Annotated protein: | Calnexin. Gene symbol: CANX. Taxonomy: Mus musculus (Mouse). Uniprot ID: P35564 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CANX |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Synapse type(s): | cerebellum |
| Annotated paper: | Li HD, et al. "Enhanced clathrin-dependent endocytosis in the absence of calnexin" PLoS One. 2011;6(7):e21678 PMID:21747946 |
| Figure(s): | Fig. 2e |
| Annotation description: | Increased endocytosis in the absence of Calnexin in cultured cells (mouse neuroblastoma) and neurons. Significantly increased numbers of synaptic vesicles in cerebellar synapses (Fig. 2e-f). Formally the authors demonstrate in non-neuronal cells that Calnexin is an involved in regulating endocytosis by inhibiting transferrin uptake (Fig. 3). From the KO phenotype one can conclude that endocytosis of synaptic vesicles is also affected. 14/3/2017 Pim - Literal: "Here we showed that the calnexin C-tail is a potent inhibitor of endocytosis in neuronal cells indicating a functional specialization of calnexin domains. " - EM: "For electron microscopy analysis, 7-day old mice were euthanized by decapitation and their cerebella were taken." |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| SynGO annotation ID: | 296 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |