| Annotated protein: | Endophilin-A1 (Endophilin-1) (SH3 domain protein 2A) (SH3 domain-containing GRB2-like protein 2) (SH3p4). Gene symbol: SH3GL2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q62420 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SH3GL2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Yang Y, et al. "Endophilin A1 regulates dendritic spine morphogenesis and stability through interaction with p140Cap" Cell Res. 2015 Apr;25(4):496-516 PMID:25771685 |
| Figure(s): | Fig.1 |
| Annotation description: | Fig.1: "Endophilin A1 localizes in dendritic shafts and spines in mature neurons" - "Confocal microscopy and structured illumination microscopy analyses indicated that the endophilin A1 clusters distributed to both dendritic shafts and spines (Figure 1B and and1C)1C) and some clusters were in close proximity to or partially colocalized with the postsynaptic density (PSD) structure labeled with antibodies to PSD95 (Figure 1C, Supplementary information, Figure S1A and S1C, and Movie S1). Consistently, subcellular fractionation assays revealed that a significant amount of endophilin A1 was distributed in the PSD fraction (Figure 1D). Moreover, in agreement with previous reports11, immunogold labeling of ultrathin sections with antibodies to endophilin A1 detected its signals in the postsynaptic spines of mouse hippocampus (Figure 1E). Live imaging of hippocampal neurons also revealed distribution of a endophilin A1-GFP fusion protein (EENA1-LentiGFP) in both axon boutons and dendritic protrusions, with fluorescent signals in both tips of filopodia and mushroom-shaped spines (Figure 1F). These data prompted us to investigate whether endophilin A1 also functions in the postsynaptic compartment." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2959 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |