| Annotated protein: | Cadherin-9 (T1-cadherin). Gene symbol: CDH9. Taxonomy: Mus musculus (Mouse). Uniprot ID: P70407 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CDH9 |
| Ontology domain: | Biological Process |
| SynGO term: | synapse adhesion between pre- and post-synapse (GO:0099560) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Basu R, et al. "Heterophilic Type II Cadherins Are Required for High-Magnitude Synaptic Potentiation in the Hippocampus" Neuron. 2017 Sep 27;96(1):160-176.e8 PMID:28957665 |
| Figure(s): | Fig.4 |
| Annotation description: | Fig.4: "Cadherin-9 Mediates Trans-cellular Adhesion via Cadherins-6 and -10" Cell aggregation assay: -"To determine whether cadherin-9 can bind in trans to other cadherins expressed in the hippocampus, we conducted cell aggregation assays (Takeichi and Nakagawa, 2001)."..."we identified four heterophilic cadherin pairs; cadherins-6/9, -9/10, -10/6, and -8/11 (Figures S4C and S4D)." - "We then expressed Cdh9-smFPFLAG in one set of CHO cells to simulate CA3 neurons and plated them with a second set of CHO cells expressing Cdh6-smFPHA, Cdh10-smFPMYC, or Cdh6-smFPHA and Cdh10-smFPMYC (Figure 4C) to simulate CA1 neurons. Immunostaining shows that cadherins localize at the interaction interfaces in all combinations tested (Figures 4C and S4E)." - "All cadherins tested were enriched in the synaptosome fraction relative to lysates by P14 (Figures 4E). " - "We co-cultured neurons expressing Cdh9-smFPFLAG with neurons expressing Cdh6-smFPHA, Cdh10-smFPMYC, or both Cdh6-smFPHA and Cdh10-smFPMYC and immunostained for epitope tags and the synaptic markers vGLUT1 and PSD95. We observed co-localization of every pairwise combination of cadherins at synapses (Figures 4G-4I). "..."all three cadherins simultaneously co-localize at synaptic sites marked by juxtaposed vGLUT and PSD95 puncta (Figure 4J)." In Fig.2 and 5 the in situ consequence of the adhesion activity for the Cadherin-6/9/10 is shown via effects on spine morphology and LTP (not annotated). |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Protein-protein interaction (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2955 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |