| Annotated protein: | Brefeldin A-inhibited guanine nucleotide-exchange protein 2 (Brefeldin A-inhibited GEP 2) (ADP-ribosylation factor guanine nucleotide-exchange factor 2). Gene symbol: ARFGEF2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q7TSU1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARFGEF2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic hippocampus, GABAergic |
| Annotated paper: | Charych EI, et al. "The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the beta subunits of the GABA receptors" J Neurochem. 2004 Jul;90(1):173-89 PMID:15198677 |
| Figure(s): | Fig.7, 8 |
| Annotation description: | Fig.7: "Subcellular distribution of BIG2 in neurons by EM immunocyto-chemistry" - "BIG2 immunolabeling is also locali zed in the postsynapticcytoplasm (f), associated with microtubules (arrowheads), near thepostsynaptic density." Fig.8: "Synaptic distribution of BI G 2 in neurons by EM immunocyto-chemistry" - "Pre-embedding EM immunoperoxidase labelingwith anti-BIG2 shows immunolabeling at the postsynaptic density(arrows) and dendritic cytoplasm of an asymmetric type I synapse on adendritic spine (a) and of a symmetric type II synapse on a dendriticshaft (b)" - "Double labe-ling experiments show that BIG2 immunolabeling (arrows , 12 nmdiameter gold particles in f and 18 nm in g and h) is also localizedpresynaptically at GABAergic synapses identified not only by thesymmetric synapse morphology but also by the presence of GABA inthe presynaptic terminal (f, arrowheads, 18 nm gold particles) orGABAARs in the postsynaptic membrane (b 2/3, g and h, arrowheads,10 nm diameter gold particles). " |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2950 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |